Effect of 650 nm diode laser on Pseudomonas aeruginosa of Ehrlich tumor

In an attempt to discover effective alternative treatment, Low Level Laser Therapy (LLLT), commonly known as photo-biostimulation or phototherapy has emerged. Traditional methods used for cancer treatment is surgery followed by chemotherapy and/or radiotherapy. Recently microbial therapy is being used to control the tumor growth. However the control of the activity of the microbe faces has several problems which limited in its applicability. Therefore, the aim of the present work was to study the possibility of controlling the activity of an aggressive microorganism, Pseudomonas Aeruginosa (Ps.) by 650 nm diode laser beam; power density 150 mW/cm, the spot size of the laser beam was 1 cm with exposure durations 30 minutes per day, incident doses of (30 Joules/cm) and treatment schedule of once/day were used in the experiments.Materials and methods: The mice were divided into 7 groups namely A, B, C, D, E, F and G. The group A was used as control. The group B injected in the right thigh by 0.2 ml from a suspension containing 10 cell /ml of the Ehrlich tumor only. The group C injected with100μL PBS which contains 4x10 CFU of Pseudomonas aeruginosa. The group D injected in the right thigh by 0.2 ml from a suspension containing 10 cells/ml of the Ehrlich tumor and exposed to Laser radiation for 30 mins /day along four successive days. The group E injected with 100μl PBS which contains 4x10 CFU of Pseudomonas aeruginosa, and exposed to Laser radiation for 30 mins /day along four successive days. The group F mice injected in the right thigh by both 0.2 ml from a suspension containing 10 cell /ml of the Ehrlich tumor and 100μL PBS which contains 4x10 CFU of Pseudomonas aeruginosa. The group G injected in the right thigh by both 0.2 ml from a suspension containing 10 cell/ml of the Ehrlich tumor and 100μL PBS which contains 4x10 CFU of Pseudomonas aeruginosa and exposed to Laser radiation for 30 mins/day along four successive days. The tumor growth characteristics were followed for all animals. Cellular changes were evaluated using cells viability (ATP production) and cytokines expression (IFNγ and IL-6) the treated and untreated tumors were studied and survival rate were demonstrated.The results: The mice injected with Ps alone caused the death of all animals till day 4. While, the mice with post tumor implantation (PI) which exposed (30 Joules/cm) of 650 nm diode laser irradiation decreased the mass tumor and that tumors infected with Ps. On the other hand, tumors infected with microorganisms then exposed (30 Joules/cm) to 650 nm diode laser for 1 day post infection showed a sudden decrease in tumor volume during next three days of infection then the tumor began to grow again with higher rate. More than 70% of the animals survive at day 35 PI. The groups A, B, C and F in the 1, 2, 3 and 4 days with 0 Joules /cm produced a non significant increase in ATP luminescences. While, the groups D, E and G irradiated once at the 1, 2, 3 and 4 day with 30 Joules /cm produced a significant increase in ATP. In addition, the groups A, B, C (0 Joules/cm) and F cells showed a significant increase in the level production of IFN-γ and IL-6 in serum. Moreover, Irradiation groups D, E and G with (30 Joules /cm) cells also produced a significant change of both cytokines, but, the group G exhibited a highly significant increase in the production of IFN-γ and IL-6 in serum more than the groups D and E. [Nashwa Abass Ahmed, Hala Moustafa Ahmed, Hanan Moustafa Rabei, Ismail Hegazy. Effect of 650 nm diode laser on Pseudomonas aeruginosa of Ehrlich tumor. J Am Sci 2012;8(12):729-738]. (ISSN: 1545-1003). http://www.americanscience.org. 102